کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3392355 | 1592683 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Detection of donor-specific HLA antibodies before and after removal of a rejected kidney transplant Detection of donor-specific HLA antibodies before and after removal of a rejected kidney transplant](/preview/png/3392355.png)
Serum analysis of patients considered for retransplantation has a potential limitation that the rejected allograft may absorb HLA antibodies. We have determined how the highly sensitive micro bead-based Luminex antibody-binding assay with single antigens can detect donor-specific HLA antibodies (DSA) in patients before and after surgical removal of a rejected allograft. This analysis was done for 65 allograft nephrectomy (allonx) cases contributed by 16 laboratories worldwide.In the HLA-A,B and -DRB1 mismatch categories the incidence of DSA reactivity pre-allonx and post-allonx was 64% vs 87% (p = 0.0033) and 57% vs 86% (p = 0.001), respectively. The frequencies of individual reactive antigens were also lower before allonx: for HLA-A,B antigens: 49% vs 75% (p < 0.0001) and DRB1 antigens: 48% vs 79% (p = 0.0001). On the other hand, no significant differences were seen between the pre-allonx and post-allonx frequencies of DSA to DRB3/4/5 (65% vs 78%, p = 0.22) and DQ mismatches (76% vs 87%, p = 0.18).Conclusion: although the sensitive Luminex antibody assay can detect anti-donor antibodies in the presence of a rejected transplant, it is apparent that the antibody specificity pattern is often incomplete especially against the HLA-A, -B and DR mismatches. This understanding seems relevant to the determination of acceptable mismatches for patients considered for retransplantation.
Journal: Transplant Immunology - Volume 22, Issues 3–4, February 2010, Pages 105–109