کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3392365 | 1592683 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Donor IL-4-treatment induces alternatively activated liver macrophages and IDO-expressing NK cells and promotes rat liver allograft acceptance
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کلمات کلیدی
FOXP3TNFPVGγcCTLA4NOS2Heme oxygenase-1IDOHO-1MSTnatural killer - (سلول های) کشنده طبیعیcytotoxic T-lymphocyte antigen 4 - آنتی ژن T-lymphocyte سیتوتوکسی 4Cho - برایChinese Hamster Ovary - تخمدان هامستر چینیIndoleamine dioxygenase - دیئولزیناز انسولینmedian survival time - زمان بقا متوسطinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییtumour necrosis factor - عامل نکروز تومورAlternative activation - فعال سازی جایگزینLiver transplantation - پیوند کبدMacrophage mannose receptor - گیرنده مانوز ماکروفاژ
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Most approaches to transplant tolerance involve treatment of the recipient to prevent rejection. This study investigates donor treatment with IL-4 for its effect on subsequent rat liver allograft survival. Rat orthotopic liver transplants were performed in rejecting (PVG donor to Lewis recipient) or spontaneously tolerant (PVG to DA) strain combinations. Donors were untreated or injected intraperitoneally with IL-4 (30,000Â U/day) for 5Â days. Tissue infiltrates and gene expression were examined by immunohistochemistry and real-time quantitative PCR. IL-4 induced a marked leukocyte infiltrate in donor livers prior to transplant. Macrophages comprised the major population, although B cells, T cells and natural killer (NK) cells also increased. IL-4-induced liver macrophages had an alternatively activated phenotype with increased expression of mannose receptor but not inducible nitric oxide synthase (NOS2). IL-4 also induced IDO and IFN-gamma expression by NK cells. Donor IL-4-treatment converted rejection to acceptance in the majority of Lewis recipients (median survival time >Â 96Â days) and did not prevent acceptance in DA recipients. Acceptance in Lewis recipients was associated with increased donor cell migration to recipient spleens and increased splenic IL-2, IFN-gamma and IDO expression 24Â h after transplantation. Donor IL-4-treatment increased leukocytes in the donor liver including potentially immunosuppressive populations of alternatively activated macrophages and IDO-expressing NK cells. Donor treatment led to long-term acceptance of most livers in association with early immune activation in recipient lymphoid tissues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplant Immunology - Volume 22, Issues 3â4, February 2010, Pages 172-178
Journal: Transplant Immunology - Volume 22, Issues 3â4, February 2010, Pages 172-178
نویسندگان
Chuanmin Wang, Szun Szun Tay, Giang T. Tran, Suzanne J. Hodgkinson, Richard D.M. Allen, Bruce M. Hall, Geoffrey W. McCaughan, Alexandra F. Sharland, G. Alex Bishop,