کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3392526 | 1221225 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibitory effects of anti-CII TA M1-RNA on IFN-γ induced major histocompatibility complex class II antigens expression on cultured human chondrocytes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inhibitory effects of anti-CII TA M1-RNA on IFN-γ induced major histocompatibility complex class II antigens expression on cultured human chondrocytes Inhibitory effects of anti-CII TA M1-RNA on IFN-γ induced major histocompatibility complex class II antigens expression on cultured human chondrocytes](/preview/png/3392526.png)
چکیده انگلیسی
Major histocompatibility complex class II (MHC-II) trans-activator (CII TA) has been shown to be required for constitutive and IFN-γ-induced MHC-II transcription. This study investigated the inhibitory effect of anti-CII TA M1-RNA on expression of MHC-II in chondrocytes in response to IFN-γ. M1-RNAs with different guide sequence (GS) recognizing 452 or 3408 sites in CII TA (M1-452-GS and M1-3408-GS, respectively) were cloned into pUC19 vector. Target mRNA (3176-3560) in CII TA was obtained from Raji cell and inserted into pGEM-7zf(+) plasmid. The recombinant M1-RNAs and their target mRNA were incubated in a cell-free condition. It showed that only M1-3408-GS could cleave the target mRNA exclusively. M1-3408-GS was also cloned into psNAV vector (named pA3408). Chondrocytes was stably transfected with pA3408 and expressions of classical MHC-II (HLA-DR, -DP, -DQ) were analyzed by Flow Cytometry. The level of CII TA mRNA was measured by RT-PCR. Peripheral blood mono-nucleated cells (PBMNCs) were stimulated by pA3408-positive chondrocytes in mixed lymphocyte reaction, and proliferation of PBMNCs and IL-2 mRNA were detected. The expression of HLA-DR and HLA-DP on pA3408-positive chondrocytes in response to IFN-γ decreased 73.00% ± 5.24%, 88.47% ± 2.02%, respectively (P < 0.05); So did the content of CII TA mRNA (70.11% ± 5.79%, P < 0.05). Proliferation of PBMNCs and production of IL-2 mRNA were both inhibited by pA3408 in mixed lymphocyte reaction. This is the first description that anti-CII TA M1-RNA could prevent IFN-γ-induced CII TA transcription and results in a decreased MHC-II expression in chondrocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplant Immunology - Volume 17, Issue 4, June 2007, Pages 231-236
Journal: Transplant Immunology - Volume 17, Issue 4, June 2007, Pages 231-236
نویسندگان
Fei He, Rong Guo, Xin Du, Ze-Sheng Lu, Jian-Yu Weng, Wei Lin,