کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3392532 | 1221225 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of blocking the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in a rat small intestinal transplantation model
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کلمات کلیدی
HEVMLNMAdCAM-1n.s.EVTGALTPODVillus height - ارتفاع ویولوزgut-associated lymphoid tissues - بافت لنفاوی وابسته به رودهHistochemical analysis - تجزیه و تحلیل هیستوشیمیLamina propria - لامینا پروپریاBlocking antibody - مسدود کردن آنتی بادیadhesion molecule - مولکول چسبندگیmucosal addressin cell adhesion molecule-1 - مولکول چسبندگی سلول مخاطی 1 مخاطیhigh endothelial venules - ونولهای اندوتلیال بالاIntestinal transplantation - پیوند رودهPeyer's patches - پیک هاmesenteric lymph nodes - گره های لنفاوی مزانتریک
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effect of blocking the expression of the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in a graft by an antibody, and immunohistochemical changes in the graft were monitored, using a rat small intestinal transplantation model. Dark Agouti (DA) rat small intestines were heterotopically transplanted into Lewis (LEW) rats. The graft was treated with or without an anti-MAdCAM-1 antibody, F(abâ²)2, during the operation. The survival of the grafts and histological changes, such as lymphocyte infiltration and destruction of the intestinal architecture in epithelium villus thickness, villus height and submucosal thickness of the graft, were examined. The expression of MAdCAM-1 and β7 integrin in the graft was also checked by immunostaining. Furthermore, graft infiltrating lymphocytes, in mesenteric lymph nodes (MLN) and Peyer's patches (PP) were measured by FACS analysis. Survival was prolonged in the DA graft with anti-MAdCAM-1 F(abâ²)2 treatment; DA to LEW: 7.0 ± 3.3, DA to LEW with the antibody: 24.6 ± 8.4 days (p < 0.05). Histological findings and scoring of the grafts were consistent with this conclusion. Moreover, MAdCAM-1 expression itself was suppressed in grafts of the antibody-treated group. While a FACS analysis showed no difference in the % of CD4+ T cells and CD8+ T cells in the PP of the graft, CD4+ T cells in the MLN of the antibody-treated graft were significantly low. A strategy directed at blocking the adhesion molecule, MAdCAM-1, in the small intestinal grafts could be useful in the prevention of acute rejection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transplant Immunology - Volume 17, Issue 4, June 2007, Pages 271-277
Journal: Transplant Immunology - Volume 17, Issue 4, June 2007, Pages 271-277
نویسندگان
Yoshiyuki Ihara, Shuji Miyagawa, Toshimichi Hasegawa, Takuya Kimura, Hengjie Xu, Masahiro Fukuzawa,