کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3395342 1221684 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Examine growth inhibition pattern and lactic acid production in Streptococcus mutans using different concentrations of xylitol produced from Candida tropicalis by fermentation
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Examine growth inhibition pattern and lactic acid production in Streptococcus mutans using different concentrations of xylitol produced from Candida tropicalis by fermentation
چکیده انگلیسی

Twenty clinical isolates of Streptococcus sp. were isolated from six clinical samples of dental caries on MSFA. Amongst these isolates, five clinical isolates were identified as S treptococcus mutans on the basis of morphological, biochemical and 16S rDNA sequencing. The isolated strains of S. mutans were exposed to fermented and purified xylitol (0.25–15.0%) and tested for its anti-microbial effects against control medium (Brain Heart Infusion without xylitol) after 12 h. The plate assay was developed using bromocresol green as an indicator dye in order to study the relative growth inhibition pattern of clinical sample at different concentrations of an anti-microbial compound in a single petriplate. The morphology of S. mutans cells in brain heart infusion (BHI) medium containing xylitol resulted in a diffused cell wall as observed using gram staining technique. The minimum inhibitory concentration (MIC) is 0.25% for S. mutans obtained from different clinical samples. The MIC50 and MIC90 is 5.0% and 10.0% xylitol respectively of the selected S. mutans being designated as clinical isolate B (6). The zone of inhibition was 72 mm and lactic acid production was 0.010 g/l at 10% xylitol concentration in Brain Heart Infusion Broth.


► Streptococcus strains were isolated and exposed to purified xylitol in the range from 0.25% to 15.0%.
► Minimum inhibitory concentration of purified and crystallized fermentative xylitol for clinical samples is 0.25%.
► Antibacterial effect of xylitol was examined by zone of inhibition, bacterial growth pattern and lactic acid production.
► Plate assay was developed using bromocresol green.
► The MIC50 and MIC90 is 5.0% and 10.0% xylitol respectively of the selected clinical isolate B (6).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Anaerobe - Volume 18, Issue 3, June 2012, Pages 273–279
نویسندگان
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