کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3395632 1221697 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An indirect ELISA for serodiagnosis of cattle footrot caused by Fusobacterium necrophorum
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
An indirect ELISA for serodiagnosis of cattle footrot caused by Fusobacterium necrophorum
چکیده انگلیسی

A serodiagnostic ELISA (rL-ELISA) using recombinant truncated leukotoxin protein PL2 (aa 311–644) of Fusobacterium necrophorum as antigen was developed for detection of antibodies against F. necrophorum from cattle footrot. In rL-ELISA, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type A, bovine Escherichia coli, and bovine Salmonella. The rL-ELISA could confirm the existence of antibodies against F. necrophorum at day 7 after infection. Detection of the field samples indicated relative sensitivity of rL-ELISA to nL-ELISA using the purified native leukotoxin A as antigen was 96.43%, and relative specificity of rL-ELISA to nL-ELISA was 94.26%. These data demonstrated the rL-ELISA would have a potential use for early diagnosis of cattle footrot caused by F. necrophorum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Anaerobe - Volume 16, Issue 4, August 2010, Pages 317–320
نویسندگان
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