کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3406818 | 1593488 | 2012 | 6 صفحه PDF | دانلود رایگان |

Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.
► Original nested primer sets for Dobrava and Puumala hantavirus detection designed.
► Primers created for S and M segment Dobrava and M segment Puumala.
► PCR assay optimized on infected cell cultures.
► Original nested primers successfully used for HFRS diagnostic in patient.
Journal: Journal of Virological Methods - Volume 182, Issues 1–2, June 2012, Pages 37–42