کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3406818 1593488 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina
چکیده انگلیسی

Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.


► Original nested primer sets for Dobrava and Puumala hantavirus detection designed.
► Primers created for S and M segment Dobrava and M segment Puumala.
► PCR assay optimized on infected cell cultures.
► Original nested primers successfully used for HFRS diagnostic in patient.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 182, Issues 1–2, June 2012, Pages 37–42
نویسندگان
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