کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3406935 1223610 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a loop-mediated isothermal amplification for visual detection of the HCLV vaccine against classical swine fever in China
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Development of a loop-mediated isothermal amplification for visual detection of the HCLV vaccine against classical swine fever in China
چکیده انگلیسی

A loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the rapid and specific detection of HCLV vaccine strain against classical swine fever. Four primers were designed for amplification of NS5B gene region with Bst DNA polymerase at a constant temperature of 65 °C. The products showed ladder-like pattern on 2% agarose gel, and can be visualised after addition of SYBR Green I dye. The detection limit of the assay was 5 copies of the HCLV genome per reaction. No cross-reaction with other porcine viruses including different wild-type CSFV strains and the bovine viral diarrhoea virus was observed. The agreement between the LAMP and TaqMan real-time RT-PCR assays was 94.4% for the detection of 72 batches of HCLV vaccine. The assay provides a rapid tool for the control of vaccine quality and can be an accompanying assay of the LAMP for wild-type CSFV described previously for differential diagnosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 171, Issue 1, January 2011, Pages 200–205
نویسندگان
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