کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416144 1225003 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle
چکیده انگلیسی

Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MβCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 9, Issue 1, January 2007, Pages 96–102
نویسندگان
, , , , ,