کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416224 1593693 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Occurrence of virulence-associated genes in Pasteurella multocida isolates obtained from different hosts
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Occurrence of virulence-associated genes in Pasteurella multocida isolates obtained from different hosts
چکیده انگلیسی


• The occurrence of ompA, ompH, oma87, sodA and sodC genes was noticed in all of the samples.
• The highest occurrence of toxA gene was noted in the small ruminants, and capsular type D isolates.
• The toxA, tbpA and pfhA genes can be considered as important epidemiological markers.

Pasteurella multocida infects a wide range of animals and the infection may spread to human through animal bites and scratches. Pasteurella multocida isolates, obtained from several clinically healthy and diseased animals (bovine, sheep, goat, poultry, dog and cat), were investigated for capsule biosynthesis (capA, B, D, E and F) and expression of 22 virulence-associated genes using Polymerase Chain Reaction (PCR). Multiplex PCR results revealed expression of capA, capD and capB genes in 81 (61.83%), 30 (22.90%) and 10 isolates (7.29%), respectively. However, neither of the isolates harbored capE or capF genes and ten isolates (7.29%) were negative for all cap genes. The expression of the capB gene was observed in small ruminant isolates. The occurrence of the ompA, ompH, oma87, sodA and sodC genes was noticed in all of the samples. More than 90% of the isolates harbored hgbA (96.18%), ptfA (95.41%), exbBD-tonB (93.12%), nanB (93.12%) and plbB genes (90.83%). The transferrin binding protein encoding gene tbpA was exclusively detected in the ruminant isolates. The limited number of isolates (25.95%) harbored dermonecrotoxin gene (toxA) and the highest occurrence was noted in the small ruminants, and the capsular type D isolates. This study highlights that the toxA, tbpA, and pfhA genes can be considered as important epidemiological markers for the characterization of P. multocida isolates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 96, July 2016, Pages 52–57
نویسندگان
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