Molecular survey of canine vector-borne diseases in stray dogs in Thailand

• A molecular survey of canine vector-borne diseases (CVBDs) in Thailand
• 43.1% samples were found to be infected with one or more pathogens.
• Mycoplasma spp. were the most prevalent of 36/181 (19.9%).
• Babesia sp., E. canis, Hepatozoon sp. and A. platys infections were also found.
• This study provides important data for the surveillance of CVBDs in Thailand.

Despite the large population of stray dogs in Thailand, there is limited information on the prevalence of canine vector-borne diseases (CVBDs). In this study, a molecular survey was conducted to determine the prevalence of Babesia spp., Ehrlichia canis, Hepatozoon spp., Anaplasma platys and Mycoplasma spp. in dogs in Thailand. Of the 181 dog blood samples tested by PCR, 78/181 (43.1%) were found to be infected with one or more pathogens. The overall prevalence rates of Mycoplasma spp., Hepatozoon spp., Babesia spp., A. platys and E. canis infections were 19.9%, 18.8%, 9.4%, 4.4% and 3.9%, respectively. To the authors' knowledge, this is the first report of Mycoplasma infection in Thailand in dogs. The current findings are important for future surveillance of CVBDs and designing appropriate approaches for diagnosis and control for the diseases in Thailand.