کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3417898 1225483 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular identification of Paragonimus species by DNA pyrosequencing technology
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Molecular identification of Paragonimus species by DNA pyrosequencing technology
چکیده انگلیسی

DNA pyrosequencing for PCR amplicons is an attractive strategy for the identification of microorganisms because of its short time performance for large number of samples. In this study, the primers targeting the fragment of ITS2 region of nuclear ribosomal RNA gene were newly developed for pyrosequencing-based identification of 6 Paragonimus species, Paragonimus bangkokensis, Paragonimus harinasutai, Paragonimus heterotremus, Paragonimus macrorchis, Paragonimus siamensis and Paragonimus westermani. Pyrosequencing determination of 39 nucleotides of partial ITS2 region could discriminate 6 Paragonimus species, and could also detect intra-species genetic variation of P. macrorchis. This DNA pyrosequencing-based identification can be a valuable tool to improve species-level identification of Paragonimus in the endemic areas.

Figure optionsDownload as PowerPoint slideHighlights
► Pyrosequencing coupled with PCR differential detecting Paragonimus spp.
► Assay yields rapid and high throughput of species-level identification.
► Intra-specific variation of Paragonimus macrorchis was found.
► Providing an exciting alternative for molecular surveillance of Paragonimus

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Parasitology International - Volume 62, Issue 3, June 2013, Pages 341–345
نویسندگان
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