کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3418161 1225496 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast
چکیده انگلیسی

Bioinformatics research on Plasmodium falciparum revealed two isoforms of pyruvate kinase: type-I and type-II enzymes. The type-I enzyme shows typical glycolytic properties, while type-II enzyme is involved in fatty acid type-II biosynthesis and has been predicted to localize to the apicoplast with the targeting signal in its N-terminus. The type-I and type-II isoforms have the same evolutionary origin as Toxoplasma gondii isozymes, TgPyKI and TgPyKII, respectively; however, TgPyKII localizes to both the mitochondrion and the apicoplast. Accordingly, we made a recombinant full length of P. falciparum pyruvate kinase type-II protein using a wheat germ cell-free expression system and obtained a specific antibody against the type-II protein. Fluorescent microscopic analysis revealed that P. falciparum type-II enzyme was localized only to the apicoplast, not to the mitochondrion. The data suggest differences in localization and metabolic pathways between P. falciparum and T. gondii pyruvate kinase isoforms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Parasitology International - Volume 58, Issue 1, March 2009, Pages 101–105
نویسندگان
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