کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3418246 1225503 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
چکیده انگلیسی

The branches and leaves of Tabernaemontana catharinensis were extracted with supercritical fluid using a mixture of CO2 plus ethanol (SFE), and the indole alkaloid enriched fraction (AF3) was selected for anti-Leishmania activity studies. We found that AF3 exhibits a potent effect against intracellular amastigotes of Leishmania amazonensis, a causative agent of New World cutaneous leishmaniasis. AF3 inhibits Leishmania survival in a dose-dependent manner, and reached 88% inhibition of amastigote growth at 100 μg/mL. The anti-parasite effect was independent of nitric oxide (NO), since AF3 was able to inhibit NO production induced by IFN-γ plus LPS. In addition, AF3 inhibited TGF-β production, which could have facilitated AF3-mediated parasite killing. The AF3 fraction obtained from SFE was nontoxic for host macrophages, as assessed by plasma membrane integrity and mitochondrial activity. We conclude that SFE is an efficient method for obtaining bioactive indole alkaloids from plant extracts. Importantly, this method preserved the alkaloid properties associated with inhibition of Leishmania growth in macrophages without toxicity to host cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Parasitology International - Volume 56, Issue 2, June 2007, Pages 135–139
نویسندگان
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