کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3424120 | 1227194 | 2013 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Canine and feline parvoviruses preferentially recognize the non-human cell surface sialic acid N-glycolylneuraminic acid Canine and feline parvoviruses preferentially recognize the non-human cell surface sialic acid N-glycolylneuraminic acid](/preview/png/3424120.png)
Feline panleukopenia virus (FPV) is a pathogen whose canine-adapted form (canine parvovirus (CPV)) emerged in 1978. These viruses infect by binding host transferrin receptor type-1 (TfR), but also hemagglutinate erythrocytes. We show that hemagglutination involves selective recognition of the non-human sialic acid N-glycolylneuraminic acid (Neu5Gc) but not N-acetylneuraminic acid (Neu5Ac), which differs by only one oxygen atom from Neu5Gc. Overexpression of α2-6 sialyltransferase did not change binding, indicating that both α2-3 and α2-6 linkages are recognized. However, Neu5Gc expression on target cells did not enhance CPV or FPV infection in vitro. Thus, the conserved Neu5Gc-binding preference of these viruses likely plays a role in the natural history of the virus in vivo. Further studies must clarify relationships between virus infection and host Neu5Gc expression. As a first step, we show that transcripts of CMAH (which generates Neu5Gc from Neu5Ac) are at very low levels in Western dog breed cells.
► Feline and canine parvoviruses recognize Neu5Gc but not Neu5Ac, which differ by one oxygen atom.
► The underlying linkage of these sialic acids does not affect recognition.
► Induced Neu5Gc expression on target cells that normally express Neu5Ac did not enhance infection.
► Thus, the conserved binding preference plays an important yet unknown role in in vivo infections.
► Population and breed variations in Neu5Gc expression occur, likely by regulating the gene CMAH.
Journal: Virology - Volume 440, Issue 1, 25 May 2013, Pages 89–96