کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3424143 | 1227197 | 2013 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Infectivity of hepatitis C virus correlates with the amount of envelope protein E2: Development of a new aptamer-based assay system suitable for measuring the infectious titer of HCV Infectivity of hepatitis C virus correlates with the amount of envelope protein E2: Development of a new aptamer-based assay system suitable for measuring the infectious titer of HCV](/preview/png/3424143.png)
Various forms of hepatitis C virus (HCV)-related particles are produced from HCV-infected cells. Measuring infectivity of a HCV sample with the conventional ‘foci counting method’ is laborious and time-consuming. Moreover, the infectivity of a HCV sample does not correlate with the amount of viral RNA that can be measured by real-time RT-PCR. Here we report a new assay suitable for quantifying infectious HCV particles using aptamers against HCV E2, which is named ‘Enzyme Linked Apto-Sorbent Assay (ELASA)’. The readout value of HCV ELASA linearly correlates with the infectious dose of an HCV sample, but not with the amount of HCV RNA. We also demonstrated that the activities of anti-HCV drugs can be monitored by HCV ELASA. Therefore, HCV ELASA is a quick-and-easy method to quantify infectious units of HCV stocks and to monitor efficacies of potential anti-HCV drugs.
► Single strand DNA aptamers against HCV E2 were obtained using advanced SELEX.
► We developed a new method named Enzyme-Linked Apto-Sorbent Assay (ELASA).
► The ELASA is suitable for detecting infectious HCV particles of various genotypes.
► The ELASA is suitable for therapeutic follow-ups of anti-HCV drug treatments.
► The infectious virus titers strongly correlated with the ELASA values.
Journal: Virology - Volume 439, Issue 1, 25 April 2013, Pages 13–22