کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424167 1227199 2013 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive
چکیده انگلیسی

Coronavirus replicase nsp4 is critical for virus-induced membrane modifications. An nsp4 mutant (N258T) of murine hepatitis virus (MHV) has been reported to be temperature-sensitive (ts) and to alter membrane targeting. We engineered and recovered all four possible codon variants of N258T in the cloned MHV-A59 background. All mutant viruses demonstrated impaired replication compared to wildtype MHV, but no nsp4 N258T mutant virus was ts, and all variants colocalized with viral protein markers for replication complexes, but not with markers for mitochondria. This study emphasizes that complete genome sequencing may be necessary, even with directed and confirmed reverse genetic mutants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 435, Issue 2, 20 January 2013, Pages 210–213
نویسندگان
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