کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424206 1227200 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Human papillomavirus infection is inhibited by host autophagy in primary human keratinocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Human papillomavirus infection is inhibited by host autophagy in primary human keratinocytes
چکیده انگلیسی

Human papillomavirus (HPV) infection is severely limited in its natural host, primary human keratinocytes. Our data show HPV infectivity in primary keratinocytes is over 100- and 1,000-fold lower than in established keratinocyte cell lines NIKS and HaCaT, respectively. Here, we show that the basal level of autophagy in primary human foreskin keratinocytes (HFKs) is higher than in immortalized keratinocytes, and that HPV16 virions significantly induce autophagy in HFKs. Interestingly, HPV16 infectivity is dramatically enhanced by knockdown of essential autophagy genes as well as biochemical inhibition of autophagy. The increase in HPV16 infectivity by autophagy inhibition is most significant in HFKs, showing an inverse correlation with basal HPV16 infectivity in HFK, NIKS, HaCaT, and 293FT cells. Further, inhibition of autophagy delays degradation of HPV16 capsid proteins during virus trafficking, indicating that host autophagy induced by HPV16 virions inhibits infection of primary keratinocytes through rapid degradation of viral capsid proteins.


► HPV16 infection is very limited in natural host primary human keratinocytes.
► HPV16 virions induce host autophagy during entry into primary keratinocytes.
► HPV16 infectivity is enhanced by biochemical and genetic inhibition of autophagy.
► Inhibition of autophagy delays HPV16 capsid degradation during entry.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 437, Issue 1, 1 March 2013, Pages 12–19
نویسندگان
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