کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424370 1227217 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetics of virus production from single cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Kinetics of virus production from single cells
چکیده انگلیسی

The production of virus by infected cells is an essential process for the spread and persistence of viral diseases, the effectiveness of live-viral vaccines, and the manufacture of viruses for diverse applications. Yet despite its importance, methods to precisely measure virus production from cells are lacking. Most methods test infected-cell populations, masking how individual cells behave. Here we measured the kinetics of virus production from single cells. We combined simple steps of liquid-phase infection, serial dilution, centrifugation, and harvesting, without specialized equipment, to track the production of virus particles from BHK cells infected with vesicular stomatitis virus. Remarkably, cell-to-cell differences in latent times to virus release were within a factor of two, while production rates and virus yields spanned over 300-fold, highlighting an extreme diversity in virus production for cells from the same population. These findings have fundamental and technological implications for health and disease.


► A new method enables measurement of virus production kinetics from single-cells.
► The method was used to study vesicular stomatitis virus production from BHK cells.
► Virus yields varied 300-fold, while latent times were within two-fold.
► Such measures reveal how traits of virus production may be distributed and correlated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 424, Issue 1, 1 March 2012, Pages 11–17
نویسندگان
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