کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3424394 | 1227218 | 2012 | 9 صفحه PDF | دانلود رایگان |
The protein kinase regulated by RNA (PKR) enhances both activation of mitogen-activated protein kinases and the induction of interferon beta (IFN-β) by measles virus defective in C-protein expression (Cko). Here we used complementation of human cell lines stably deficient in PKR (PKRkd) to probe the basis of these PKR-mediated responses. We found that PKRkd HeLa and amnion U cell lines were defective for virus-mediated activation of IFN induction signaling components compared to PKR-sufficient control cells. Complementation of PKRkd cells with wildtype PKR, but not with PKR mutants defective in either catalytic activity or dsRNA-binding activity, restored JNK, p38 and ATF-2 phosphorylation and enhanced IFN-β induction following infection. By contrast to mammalian PKR, the Z-DNA binding domain-containing fish homologue of PKR, PKZ, lacked the capacity to enhance Cko virus-mediated IFN-β induction. Furthermore, inhibition of virus growth was observed with Cko-infected PKRkd cells complemented with PKR but not with PKZ.
Journal: Virology - Volume 427, Issue 2, 5 June 2012, Pages 208–216