کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424659 1227237 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dynamic association of gammaherpesvirus DNA with core histone during de novo lytic infection of primary cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Dynamic association of gammaherpesvirus DNA with core histone during de novo lytic infection of primary cells
چکیده انگلیسی

Association of herpesvirus DNA with histones has important implications for lytic and latent infections; thus herpesviruses arbitrate interactions with histones to productively infect host cells. While regulation of alpha and betaherpesvirus chromatin during lytic infection has been actively investigated, very little is known about interaction of gammaherpesvirus DNA with histones upon de novo lytic infection. Murine gammaherpesvirus-68 (MHV68) is a rodent pathogen that offers a tractable system to study gammaherpesvirus lytic infection in primary cells. In this study we report that MHV68 promoter and orilyt sequences underwent dynamic association with histone H3 during de novo lytic infection of primary macrophages and fibroblasts. Similar to HSV-1, the degree of MHV68 DNA association with histone H3 was dependent on the multiplicity of infection and was further regulated by viral DNA synthesis. Our work sets a precedent for future studies of gammaherpesvirus chromatin during de novo lytic infection.


► Gammaherpesviral DNA associates with histone H3 in lytic infection of primary cells.
► Dynamics of histone association dependent on multiplicity of infection.
► Viral DNA synthesis regulates histone H3 association.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 421, Issue 2, 20 December 2011, Pages 167–172
نویسندگان
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