کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424811 1227248 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo analysis of the cell cycle dependent association of the bovine papillomavirus E2 protein and ChlR1
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
In vivo analysis of the cell cycle dependent association of the bovine papillomavirus E2 protein and ChlR1
چکیده انگلیسی

It has been shown that the genomes of episomally maintained DNA viruses are tethered to host cell chromosomes during cell division, facilitating maintenance in dividing cells. The papillomavirus E2 protein serves this mechanism of viral genome persistence by simultaneously associating with chromatin and the viral genome during mitosis. Several host cell proteins are reported to be necessary for the association of E2 with chromatin including the cohesion establishment factor ChlR1. Here we use fluorescence resonance energy transfer (FRET) technology to confirm the interaction between BPV-1 E2 and ChlR1. Furthermore, we use synchronised live cells to study the temporal nature of this dynamic protein interaction and show that ChlR1 and E2 interact during specific phases of the cell cycle. These data provide evidence that the association of E2 with ChlR1 contributes to a loading mechanism during DNA replication rather than direct tethering during mitotic division.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 414, Issue 1, 25 May 2011, Pages 1–9
نویسندگان
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