کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424944 1227261 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Importance of the major splice donor and redefinition of cis-acting sequences of gutless feline foamy virus vectors
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Importance of the major splice donor and redefinition of cis-acting sequences of gutless feline foamy virus vectors
چکیده انگلیسی

Foamy virus vectors are potent alternatives to lenti- and gamma-retroviral vectors for gene therapy. To construct and optimize gutless feline foamy virus (FFV) replication-deficient (RD) vectors, viral elements essential for optimal efficient marker gene transduction were characterized and fine-mapped and packaging clones constructed. For these purposes, new Gag and Pol expression clones which allow efficient expression of packaging proteins and vectors carrying deletions in coding and non-coding regions of the genome were constructed and functionally evaluated. These studies demonstrate that the 5′ major splice donor (5′ SD) is indispensable for RD vectors while defined mutations introduced to inactivate the gag start codon improve transgene delivery efficiency. Based on these findings, new gutless FFV vectors were generated yielding un-concentrated vector titers above 105 transducing units (TU)/ml. By minimizing the second cis-acting sequence in the pol gene, only 3.8 kb viral sequences are maintained in the novel gutless FFV RD vectors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 394, Issue 2, 25 November 2009, Pages 208–217
نویسندگان
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