کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3425021 1227264 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interferon regulatory factor (IRF)-2 activates the HPV-16 E6–E7 promoter in keratinocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Interferon regulatory factor (IRF)-2 activates the HPV-16 E6–E7 promoter in keratinocytes
چکیده انگلیسی

Interferon regulatory factors (IRFs) are critical mediators of gene expression, cell growth and immune responses. We previously demonstrated that interferon (IFN) induction of early viral transcription and replication in several mucosal HPVs requires IRF-1 binding to a conserved interferon response element (IRE). Here we show that the IRF-2 protein serves as a baseline transactivator of the HPV-16 major early promoter, P97. Cotransfections in IRF knockout cells confirmed that basal HPV-16 promoter activity was supported by both IRF-1 and IRF-2 complexes interacting with the promoter-proximal IRE in a dose-dependent manner. Furthermore, HPV-16 E7 expression downregulates the IRF-2 promoter, thus linking IRF-2 levels to viral transforming gene expression through a negative feedback mechanism. Taken together, these observations reveal a complex viral strategy utilizing multiple signal transduction pathways during the establishment and maintenance of HPV persistence.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 399, Issue 2, 10 April 2010, Pages 270–279
نویسندگان
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