کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3425827 1594268 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics
چکیده انگلیسی

D RNA 3Yα (D3Yα), a defective (D) RNA 3 derived from the Y strain of cucumber mosaic virus (CMV-Y), was further characterized in combination with different helper viruses in the genus Cucumovirus. Interestingly, Nicotiana benthamiana plants inoculated with CMV-D8 and D3Yα developed systemic symptoms which were different from those induced by CMV-D8. To elucidate the potential effects of D RNA 3 on virus infection on the basis of the original combination of CMV-Y and D3Yα, a point mutation was made in the coat protein gene, which determined symptoms, of either CMV-Y RNA 3 (Y3) or D3Yα. Symptoms induced on N. benthamiana and N. tabacum plants, and semi-quantitative RT-PCR revealed that the ratio of RNA 3 to D RNA 3 was associated with the differences of symptoms in the leaf tissues. Furthermore, analysis of in situ hybridization suggested that there were spatial effects between coat proteins of Y3 and D3Yα in the infected leaves.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 389, Issues 1–2, 20 June–5 July 2009, Pages 59–65
نویسندگان
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