کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3426770 1227344 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A mature and fusogenic form of the Nipah virus fusion protein requires proteolytic processing by cathepsin L
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
A mature and fusogenic form of the Nipah virus fusion protein requires proteolytic processing by cathepsin L
چکیده انگلیسی

The Nipah virus fusion (F) protein is proteolytically processed to F1 + F2 subunits. We demonstrate here that cathepsin L is involved in this important maturation event. Cathepsin inhibitors ablated cleavage of Nipah F. Proteolytic processing of Nipah F and fusion activity was dramatically reduced in cathepsin L shRNA-expressing Vero cells. Additionally, Nipah virus F-mediated fusion was inhibited in cathepsin L-deficient cells, but coexpression of cathepsin L restored fusion activity. Both purified cathepsin L and B could cleave immunopurified Nipah F protein, but only cathepsin L produced products of the correct size. Our results suggest that endosomal cathepsins can cleave Nipah F, but that cathepsin L specifically converts Nipah F to a mature and fusogenic form.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 346, Issue 2, 15 March 2006, Pages 251–257
نویسندگان
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