کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427046 1227357 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA immunization using a non-viral promoter
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
DNA immunization using a non-viral promoter
چکیده انگلیسی

Most DNA vaccines rely on strong viral promoters to optimize levels of transgene expression. Some studies have demonstrated that the potency of viral promoters does not necessarily correlate with DNA vaccine efficacy in vivo. This has partly been attributed to downregulation of these promoters by cytokines such as interferon γ induced by the CpG motives of these vaccines. In an attempt to avoid downregulation of viral promoters by IFN-γ, we tested vaccine vectors driven by the MHC class II promoter. To enhance the activity of this promoter, another plasmid expressing the human MHC class II transactivator driven by a viral promoter, the native IFN-γ inducible CIITA type IV promoter (PIV) or a synthetic promoter containing IFN-γ inducible elements was co-inoculated. Our data show that a non-viral promoter such as the MHC class II promoter tested in this study can indeed be used in DNA vaccines.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 344, Issue 2, 20 January 2006, Pages 412–420
نویسندگان
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