کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427455 1227395 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Matching complementing functions of transformed cells with stable expression of selected viral genes for production of E1-deleted adenovirus vectors
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Matching complementing functions of transformed cells with stable expression of selected viral genes for production of E1-deleted adenovirus vectors
چکیده انگلیسی

Production of E1-deleted adenovirus (rAd) vectors requires complementation by E1A and E1B functions provided by the production cell line. The two cell lines most commonly used for production of rAd vectors, 293 and Per.C6, were derived from human primary cells and contain contiguous E1A and E1B sequences from the Ad genome. As an alternative system, we tested complementation of rAd vectors using sequential transfection of individual E1A and E1B expression cassettes into A549 human lung tumor cells, which support highly efficient replication of wild type adenovirus. We found that E1A function could be complemented in A549 cells by the mutant E1Adl01/07, and that E1B function could be provided in such cells using only the 55K E1B gene. Production yields in the resulting producer cell line, designated SL0003, were similar to those obtained from 293 cells without generation of detectable recombinant replication competent adenovirus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 345, Issue 1, 5 February 2006, Pages 220–230
نویسندگان
, , , , , , , , , , , ,