کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427660 1227435 2006 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Caspase-3 and -7 mediate apoptosis of human Chang's conjunctival cells induced by enterovirus 70
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Caspase-3 and -7 mediate apoptosis of human Chang's conjunctival cells induced by enterovirus 70
چکیده انگلیسی

Enterovirus 70 (EV70) is the major etiological agent of acute hemorrhagic conjunctivitis (AHC). EV70 m.o.i.- (multiplicity of infection) and time-dependently induced apoptosis in human Chang's conjunctival (HCC) cells. UV- or heat-inactivated EV70 did not induce apoptosis. EV70-induced apoptosis was inhibited by cycloheximide and methoxysuccinyl–Ala–Ala–Pro–Val–chloromethylketone (MPCMK), but not actinomycin D and guanidine·HCl (although guanidine·HCl inhibited the apoptosis induced by EV70 infection at 0.5 PFU/cell for 18 h). EV70 infection induced activation of caspase-3 and -7 and degradation of the constitutively activated caspase-6. EV70-induced apoptotic DNA ladders and activated caspase-3 and -7, correlated with virus release. Caspase inhibitor IX (Z-VD-FMK) inhibited EV70-induced apoptosis and virus release, but not intracellular viral production. The results suggest that infectious virus and the syntheses of viral proteins especially EV70 proteases, but not viral genome RNA, are required for caspase-3 and -7-mediated EV70-induced apoptosis, and that apoptosis through cell lysis promotes EV70 release from HCC cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 347, Issue 2, 10 April 2006, Pages 307–322
نویسندگان
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