کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3427975 1594346 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ribosomal protein L4 interacts with viral protein VP3 and regulates the replication of infectious bursal disease virus
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Ribosomal protein L4 interacts with viral protein VP3 and regulates the replication of infectious bursal disease virus
چکیده انگلیسی


• IP-coupled MS method was used to screen host proteins interacting with VP3 of IBDV.
• RPL4 was identified as a new interacting partner of VP3 protein.
• The interaction of VP3 and RPL4 is involved in regulating the replication of IBDV.

VP3 protein is a structural protein which plays important roles in the virus assembly and the inhibition of antiviral innate immunity of infectious bursal disease virus (IBDV). To explore the potential roles of VP3 in the interplay of IBDV with the host cell, an immunoprecipitation (IP)-coupled mass spectra (MS) screening was performed and the host cellular ribosomal protein L4 (RPL4) was identified as a putative interacting partner of VP3 protein. The interaction of RPL4 with VP3 was further confirmed by co-immunoprecipitation (co-IP) and their colocalization in DF1 cells were observed by confocal microscopy. In addition, knockdown of RPL4 in DF1 cells resulted in reductions of the viral protein pVP2 expression and the virus titers, which reveals a significant role of RPL4 in IBDV replication. Taken together, we indicated for the first time that ribosomal protein L4 (RPL4) was an interacting partner of VP3 and involved in the modulation of IBDV replication. The present study contributes to further understanding the pathogenic mechanism of IBDV.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 211, 4 January 2016, Pages 73–78
نویسندگان
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