Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus

• We developed a transgenic mouse model expressing porcine APN that susceptible to porcine coronavirus infection.
• We generated two transgenic mouse lines expressing porcine APN in various organs.
• As they expressed porcine APN, the mice became susceptible to infection by porcine epidemic diarrhea virus, one of the porcine coronaviruses.
• These transgenic mice will be an important tool for research into the porcine coronaviruses.

Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is susceptible to porcine coronavirus infection. The porcine APN transgene was constructed by fusing with mouse proximal APN promoter at 5′ terminus and bovine growth hormone polyadenylation site at its 3′ terminus. After screen on pubs from the microinjected mice, we confirmed two transgenic lines expressing porcine APN in various organs. We confirmed the susceptibility to porcine epidemic diarrhea virus, one of the porcine coronaviruses. These transgenic mice will be an important tool for research into the porcine coronaviruses.