کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3430365 1228273 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nonclassical MHC-I and Japanese encephalitis virus infection: Induction of H-2Q4, H-2T23 and H-2T10
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Nonclassical MHC-I and Japanese encephalitis virus infection: Induction of H-2Q4, H-2T23 and H-2T10
چکیده انگلیسی

Nonclassical MHC Class 1b antigens differ from classical MHC class 1a antigens in having a restricted polymorphism as well as varied surface expression in different cell types. They have been hypothesized to play a role in bridging adaptive and innate immune responses. We examined the effects of JEV infection on the expression of classical MHC class 1a and nonclassical MHC class 1b genes in five different cell lines. Among the nonclassical genes, H-2Q4 was induced in H-6 hepatoma, primary astrocytes, mouse embryo fibroblasts, L929 and 3T3 cells. H-2T23 and H-2T10 genes were not induced in H-6 and 3T3, respectively, but were induced in the other cell lines examined. Both H-2Q4 encoded Qb1 and H-2T23 encoded Qa-1b antigens were induced on the cell surface upon JEV infection in primary astrocytes and mouse embryonic fibroblasts. Classical MHC-I genes and the genes associated with antigen presentation such as Tap1, Tap2, Tapasin, Lmp2, Lmp7 and Lmp10 as well as type 1 (α/β) IFNs were induced in all cell lines. However, IFNγ was not induced. Further, induction of H-2Q4 and H-2T23 by JEV was independent of NF-κB but type 1 IFN dependent while H-2T10 was dependent on NF-κB and type 1 IFN independent. Thus, while classical MHC genes were induced by JEV in all cell lines tested despite high levels of constitutive expression in L929 and 3T3, nonclassical genes were not inducible in all cell lines tested and involved different mechanisms of induction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 133, Issue 2, May 2008, Pages 239–249
نویسندگان
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