کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
35586 | 45097 | 2008 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Acetylation of vitamin E by Candida antarctica lipase B immobilized on different carriers Acetylation of vitamin E by Candida antarctica lipase B immobilized on different carriers](/preview/png/35586.png)
We describe for the first time the enzymatic acylation of the phenolic group of tocopherols (vitamin E) by transesterification with vinyl acetate in 2-methyl-2-butanol (2M2B). Out of 15 hydrolases screened, only the lipase B from Candida antarctica (Novozym 435) catalyzed the acylation. The acetylation of δ-tocopherol was faster than that of α-tocopherol, probably due to its lower methylation degree. A series of experiments using (R)-Trolox and p-cresol as competitive acceptors of tocopherols showed that reaction rate notably diminished when increasing acceptor size. To maximize the potential of this reaction, three immobilization carriers for C. antarctica lipase B were studied: the ion-exchange resin Lewatit (the support in Novozym 435), a biodegradable polymer (Purasorb) and polypropylene (Accurel EP100). The acetylation of α-tocopherol was faster with the enzyme immobilized in polypropylene, which was correlated with its higher porosity. A mixture hexane/2M2B 90:10 (v/v) was found to be the optimum medium composition, as it represents a compromise between substrates solubility and biocatalyst efficiency. The acylation process was no enantioselective, probably due to the fact that the chiral centers are separated from the phenolic group by a minimum of six bonds.
Journal: Process Biochemistry - Volume 43, Issue 2, February 2008, Pages 145–153