Amyloid oligomer detection by immobilized molecular chaperone

Amyloid β (Aβ) oligomer is a known causative agent of Alzheimer's disease (AD). For early recognition of AD, development of an Aβ oligomer detection system is vital. We developed a novel detection system using the molecular chaperone, Prefoldin (PFD), which can specifically interact with amyloid oligomers. Aβ oligomer detection was achieved with a PFD-coated microplate, following the enzyme-linked immunosorbent assay (ELISA) technique. The results suggest that this system specifically detects Aβ oligomers at their physiological concentrations. The problematic nonspecific adsorption of Aβ to the microplate can be effectively blocked with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer. This novel Aβ oligomer detection method may be applied for AD early recognition.

► Novel detection system of amyloid β (Aβ) oligomer which is known as causative agent of Alzheimer's disease (AD) is developed.
► Aβ oligomer detection is successfully achieved using a microplate covered with molecular chaperone which can interact with Aβ oligomer.
► We also demonstrate that at Aβ physiological concentrations this system specifically detects Aβ oligomers.