Science-based bioprocess design for filamentous fungi

Industrial bioprocesses are commonly based on empiricism rather than scientific process understanding. In this review, we summarize current strategies for science-based bioprocess design and control for filamentous fungi aiming at reducing development times and increasing process economics. We discuss recent developments and trends regarding three crucial aspects throughout the bioprocess life cycle of filamentous fungi, namely (i) strain and inoculum characterization, (ii) morphology, and (iii) rheology, as well as their effects on process performance. Complex interconnections between strain, inoculum, morphology, rheology, and process design are outlined and discussed. Only combining different hard type sensors with soft sensor technology and the development of simplified mechanistic models can enable science-based bioprocess design for filamentous fungi.

► One-stop-encyclopedia for approaches to major obstacles in filamentous bioprocesses
► Review of links between strain, inoculum, morphology, rheology, and performance
► Outline for science-based bioprocess characterization, development, and control
► Systematic bioprocess design methods for optimized industrial biotechnology.