کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3764 187 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A facile pretreatment method for efficient immobilization of penicillin G acylase
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A facile pretreatment method for efficient immobilization of penicillin G acylase
چکیده انگلیسی

A facile pretreatment method was developed to obtain high specific activity of PGA from recombinant E. coli cells for preparing immobilized enzyme with high activity, which coupled selective extraction of PGA with butyl acetate and adsorption of butyl acetate with active carbon. Butyl acetate (5%, v/v) led to a 92.0% release of PGA, the specific activity of which in the extracting solution was twice that obtained by sonication. The negative effect on PGA immobilization due to residual butyl acetate in the extracting solution was removed by adding active carbon (8%, w/v). The final PGA solution meets requirement for industrial immobilization while eliminating the high cost of a traditional purification process. This pretreatment method developed in this work is simple, highly performing and cost-effective. It has been employed in a 10 tons/year immobilized PGA production line.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 56, Issues 1–2, 15 September 2011, Pages 17–22
نویسندگان
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