Photophysical characterisation and studies of the effect of palladium(II) 5,10,15,20-tetrakis-(4-sulfonatophenyl)-porphyrin on isometric contraction of isolated human mesenteric artery: Good news for photodynamic therapy

• The formation of the triplet states of PdTSPP and the singlet oxygen were studied.
• Effect of PdTSPP on contractility of isolated human mesenteric artery was assessed.
• An efficient generation of singlet oxygen O2(1Δg) by PdTSPP was observed.
• PdTSPP had no effect on contractile properties of human mesenteric artery in vitro.

SummaryBackgroundConsidering the important roles of porphyrins in biological systems and their promising use in photodynamic therapy (PDT), the present work investigated the photophysical properties of palladium(II) 5,10,15,20-tetrakis-(4-sulfonatophenyl)-porphyrin (PdTSPP) and the effects of non-activated by light form of this porphyrin on contractile behaviour of isolated healthy endothelium-denuded human mesenteric arteries.MethodsThe photophysical characterisation of PdTSPP: the formation of the triplet states and the singlet oxygen were studied using laser flash photolysis.The effect of PdTSPP on the isometric contraction of artery segments from human mesentery was assessed utilising the precise method of artery isometric tension recording using Mulvany–Halpern wire myograph.ResultsWe found that PdTSPP had a high lifetime of the triplet states τT = 270 μs. The calculated Stern Volmer rate constant kq = 1.7 × 109 M−1 s−1 showed an efficient quenching by oxygen that indicated formation of singlet oxygen, O2(1Δg). The photophysical parameters of PdTSPP, in particular its ability to generate O2(1Δg) has defined it as an exceptionally interesting molecule for PDT.The results of the contraction study showed that PdTSPP applied in increasing concentrations (1–100 μM) had no effect on the basal tone of human mesenteric artery under isometric condition. Furthermore, PdTSPP failed to potentiate or to attenuate the isometric contraction of the artery preparations precontracted with high extracellular potassium (42 mM KCl) or with 1 nM endothelin-1.ConclusionsThe excellent photophysical properties of PdTSPP as well as the lack of an effect on the contractility of human vasculature in vitro characterise PdTSPP as a suitable compound for potential medical applications.