کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4011582 1261153 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heat shock induces apoptosis through reactive oxygen species involving mitochondrial and death receptor pathways in corneal cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
Heat shock induces apoptosis through reactive oxygen species involving mitochondrial and death receptor pathways in corneal cells
چکیده انگلیسی

Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of infrared radiation on ocular disease. In addition to photons, heat is generated as a consequence of infrared irradiation, and heat shock is widely considered to be an environmental stressor. Here, we are the first to investigate the biological effect of heat shock on Statens Seruminstitut Rabbit Cornea (SIRC) cells. Our results indicate that heat shock exhibits effective cell proliferation inhibition by inducing apoptosis. Heat shock triggers the mitochondrial apoptotic pathway indicated by a change in Bax/Bcl-2 ratios, resulting in caspase-9 activity. In addition, heat shock triggered the death receptor apoptotic pathway indicated by a change in Fas ligand expression, resulting in caspase-8 activity. Furthermore, we also found that generation of reactive oxygen species (ROS) is a critical mediator in heat shock-induced apoptosis. In addition, the antioxidant vitamin C significantly decreased heat shock-mediated apoptosis. Taken together, these findings suggest a critical role for ROS involving mitochondrial and death receptor pathways in heat shock-mediated apoptosis of cornea cells.


► Heat shock exhibits effective cell proliferation inhibition by inducing apoptosis.
► Heat shock triggers the mitochondrial and death receptor apoptotic pathways.
► Generation of ROS is a critical mediator in heat shock-induced apoptosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 93, Issue 4, October 2011, Pages 405–412
نویسندگان
, , , , , ,