کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4292 | 219 | 2009 | 8 صفحه PDF | دانلود رایگان |
Study on potential of different agro-industrial waste residues for supporting α-amylase production by a thermophilic strain of Bacillus subtilis DM-03 in solid-state fermentation (SSF) was initiated and potato peel was found to be the best substrate for α-amylase production. Linear multiple regression analysis showed that higher starch contents of the fermentable substrates enhanced the α-amylase synthesis while the higher sugar content of the substrates imposed a negative effect presumably due to catabolic repression of the enzyme synthesis. Furthermore, C/N ratio of the substrates also played a critical role in induction of α-amylase synthesis. The result of batch fermentation showed 532 ± 5 U of α-amylase production per gram of dry substrate by B. subtilis DM-03 post-72 h of incubation under optimized SSF condition. The crude α-amylase enzyme from B. subtilis DM-03 strain demonstrated excellent stability and compatibility with the laundry detergents that favors its inclusion in commercial laundry detergent formulations.
Journal: Biochemical Engineering Journal - Volume 43, Issue 2, 15 February 2009, Pages 149–156