کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4294 | 219 | 2009 | 6 صفحه PDF | دانلود رایگان |
A fed-batch process was developed for intracellular production of recombinant TATm-survivin(T34A) in Escherichia coli under the control of T7 promoter. The effects of induction mode and nutritional conditions were investigated. Compared to the one-point addition of inducer, the step-wise addition of isopropyl beta-d-thiogalactopyranoside (IPTG) maintained higher plasmid stability and increased the production level by 52%. Insufficient glucose supply after induction was observed to control acetate accumulation effectively and improved the expression of the target gene evidently. Remarkably, the pre-induction supplement of inorganic nitrogen source had a positive influence on the production of TATm-survivin(T34A). High ammonium concentration of 4.8 g l−1 was the most efficient in enhancing the production level (as the percentage of total cellular protein) and the specific productivity of TATm-survivin(T34A). As a result, the production of TATm-survivin(T34A) was optimized from 11.6% to 36.8% of total cellular protein (corresponding to 1.68 g l(1). The findings provide valuable information for optimization of recombinant protein expression.
Journal: Biochemical Engineering Journal - Volume 43, Issue 2, 15 February 2009, Pages 163–168