کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4339562 | 1295760 | 2010 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential distribution of exchange proteins directly activated by cyclic AMP within the adult rat retina
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کلمات کلیدی
dNTPBCACNBpKaPFAH89ECLEpacGCLGLASTPDEOPLCTBP2C-terminal binding protein 2Cy3VGLUT1TBSTONLIPLINLHCNCy2IS/OSRAPCNGFITCGTPIndocarbocyanineindodicarbocyaninePVDFRT-PCRmRNACy5GEFPNAHRPPKCαkDaCTBcGMPcyclic AMP - AMP cycliccAMP - cAMPcDNA - cDNAcomplimentary DNA - DNA مجانیmessenger RNA - RNA messengerpeanut agglutinin - آگلوتینین بادام زمینیImmunohistochemistry - ایمونوهیستوشیمیenhanced chemiluminescence - بهبود شیمیایی لومنbicinchoninic acid - بیسینکنینیک اسیدdeoxyribonucleotide triphosphates - تری فسفاتهای deoxyribonucleotideGDP - تولید ناخالص ملیvesicular glutamate transporter 1 - حمل کننده گلوتامات وزیکولار 1Cyanine - سیانینGuanine exchange factor - فاکتور تبادل گوانینPhosphodiesterase - فسفو دی استرازouter plexiform layer - لایه بیرونی بیرونیouter nuclear layer - لایه بیرونی هسته ایinner plexiform layer - لایه داخلی لایهinner nuclear layer - لایه داخلی هسته ایganglion cell layer - لایه سلول گانگلیونیcyclic guanosine monophosphate - مونوفسفات گوانوزین چرخه ایreverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوسparaformaldehyde - پارافرمالدهیدHorseradish peroxidase - پراکسیداز هوررادیشExchange protein directly activated by cAMP - پروتئین تبادل شده به طور مستقیم توسط cAMP فعال می شودprotein kinase A - پروتئین کیناز APolyvinylidene fluoride - پلی وینیلیدین فلورایدChAT - چتCyclic nucleotide gated channel - کانال نوکلئوتید چرخه ایcholera toxin B subunit - کلسترول توکسین B واحدkilodaltons - کیلودالتونGlutamine synthetase - گلوتامین سنتتازGuanosine triphosphate - گوانوزین تری فسفاتguanosine diphosphate - گوانوزین دی فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Differential distribution of exchange proteins directly activated by cyclic AMP within the adult rat retina Differential distribution of exchange proteins directly activated by cyclic AMP within the adult rat retina](/preview/png/4339562.png)
چکیده انگلیسی
The recently discovered exchange protein directly activated by cAMP (Epac), a guanine exchange factor for the G-protein RAP-1, is directly activated by cAMP independently of protein kinase A (PKA). While cAMP is known to be an important second messenger in the retina, the presence of Epac has not been investigated in this tissue. The goal of the present study was to determine if the Epac1 and Epac2 genes are present and to characterize their location within the retina. Western blot analysis revealed that Epac1 and Epac2 proteins are expressed within the retina, and the presence of mRNA was demonstrated with the aid of reverse transcriptase polymerase chain reaction (RT-PCR). Additionally, we used immunofluorescence and confocal microscopy to demonstrate that Epac1 and Epac2 have overlapping as well as unique distributions within the retina. Both are present within horizontal cells, rod and cone bipolar cells, cholinergic amacrine cells, retrograde labeled retinal ganglion cells, and Müller cells. Uniquely, Epac2 was expressed by cone photoreceptor inner and outer segments, cell bodies, and synaptic terminals. In contrast, Epac1 was expressed in vesicular glutamate transporter 1 (VGlut1) and C-terminal binding protein 2 (CtBP2) positive photoreceptor synaptic terminals. Together, these results provide evidence that Epac1 and Epac2 are differentially expressed within the retina and provide the framework for further functional studies of cAMP pathways within the retina.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 165, Issue 3, 3 February 2010, Pages 955-967
Journal: Neuroscience - Volume 165, Issue 3, 3 February 2010, Pages 955-967
نویسندگان
C.M. Whitaker, N.G.F. Cooper,