کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4340591 | 1295803 | 2008 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
μ-Opioid agonists inhibit the enhanced intracellular Ca2+ responses in inflammatory activated astrocytes co-cultured with brain endothelial cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSHHBSSLIFLPSDMEMDPDPETLR4SDSCGRPIL-1βBSA - BSADAMGO - DREAMDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbecco[Ca2+]i - [Ca2 +] iδ-Opioid receptor - δ-گیرنده اپیوئیدAstrocyte - آستروسیتbovine serum albumin - آلبومین سرم گاوOpioids - اپیوئیدInterleukin-1β - اینترلوکین-1βDOR - دردBlood–brain barrier - سد خونی مغزیBBB - سد خونی مغزیsodium dodecyl sulfate - سدیم دودسیل سولفاتEndothelial cells - سلولهای اندوتلیالperipheral nervous system - سیستم عصبی پیرامونیleukemia inhibitory factor - عامل مهارکننده لوکمیphosphate buffer saline - فسفات بافر شورlipopolysaccharide - لیپوپلی ساکاریدKOR - وقتیcalcitonin gene-related peptide - پپتید مرتبط با ژن کلسی تونینPNS - کارمندان دولتCalcium - کلسیمintracellular free calcium - کلسیم آزاد داخل سلولیκ-opioid receptor - گیرنده κ-اپوئیدToll-like receptor 4 - گیرنده تله مانند 4
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In order to imitate the in vivo situation with constituents from the blood-brain barrier, astrocytes from newborn rat cerebral cortex were co-cultured with adult rat brain microvascular endothelial cells. These astrocytes exhibited a morphologically differentiated appearance with long processes. 5-HT, synthetic μ-, δ- or κ-opioid agonists, and the endogenous opioids endomorphin-1, β-endorphin, and dynorphin induced higher Ca2+ amplitudes and/or more Ca2+ transients in these cells than in astrocytes in monoculture, as a sign of more developed signal transduction systems. Furthermore, stimulation of the co-cultured astrocytes with 5-HT generated a pronounced increase in intracellular Ca2+ release in the presence of the inflammatory or pain mediating activators substance P, calcitonin gene-related peptide (CGRP), lipopolysaccharide (LPS), or leptin. These Ca2+ responses were restored by opioids so that the δ- and κ-opioid receptor agonists reduced the number of Ca2+ transients elicited after incubation in substance P+CGRP or leptin, while the μ- and δ-opioid receptor agonists attenuated the Ca2+ amplitudes elicited in the presence of LPS or leptin. In LPS treated co-cultured astrocytes the μ-opioid receptor antagonist naloxone attenuated not only the endomorphin-1, but also the 5-HT evoked Ca2+ transients. These results suggest that opioids, especially μ-opioid agonists, play a role in the control of neuroinflammatory activity in astrocytes and that naloxone, in addition to its interaction with μ-opioid receptors, also may act through some binding site on astrocytes, other than the classical opioid receptor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 155, Issue 4, 9 September 2008, Pages 1237-1249
Journal: Neuroscience - Volume 155, Issue 4, 9 September 2008, Pages 1237-1249
نویسندگان
E. Hansson, A. Westerlund, U. Björklund, T. Olsson,