کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4340733 | 1295808 | 2008 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Localization of electrogenic Na/bicarbonate cotransporter NBCe1 variants in rat brain
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کلمات کلیدی
UABPBSPHINBCMAP2NeuNORFPFAHRPExtracellular K+ - K + غیر سلولیExtracellular pH - pH خارج سلولیIntracellular pH - PH درون سلولیTransporter - حمل کنندهBicarbonate transporter - حمل کننده بی کربناتUniversity of Alabama at Birmingham - دانشگاه آلاباما در بیرمنگامRoom temperature - دمای اتاقdigoxigenin - دیگوکسین ژنendoplasmic reticulum - شبکه آندوپلاسمی DIG - شماpho - فوopen reading frame - قاب خواندن بازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCerebellum - مخچهNeuron - نورونneuronal nuclei - هسته های نورونیHippocampus - هیپوکامپ paraformaldehyde - پارافرمالدهیدHorseradish peroxidase - پراکسیداز هوررادیشmicrotubule-associated protein - پروتئین مرتبط با میکروتوبولolfactory bulb - پیاز بویاییGlia - یاختههای گلیال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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![عکس صفحه اول مقاله: Localization of electrogenic Na/bicarbonate cotransporter NBCe1 variants in rat brain Localization of electrogenic Na/bicarbonate cotransporter NBCe1 variants in rat brain](/preview/png/4340733.png)
چکیده انگلیسی
The activity of HCO3â transporters contributes to the acid-base environment of the nervous system. In the present study, we used in situ hybridization, immunoblotting, immunohistochemistry, and immunogold electron microscopy to localize electrogenic Na/bicarbonate cotransporter NBCe1 splice variants (-A, -B, and -C) in rat brain. The in situ hybridization data are consistent with NBCe1-B and -C, but not -A, being the predominant NBCe1 variants in brain, particularly in the cerebellum, hippocampus, piriform cortex, and olfactory bulb. An antisense probe to the B and C variants strongly labeled granule neurons in the dentate gyrus of the hippocampus, and cells in the granule layer and Purkinje layer (e.g. Bergmann glia) of the cerebellum. Weaker labeling was observed in the pyramidal layer of the hippocampus and in astrocytes throughout the brain. Similar, but weaker labeling was obtained with an antisense probe to the A and B variants. In immunoblot studies, antibodies to the A and B variants (αA/B) and C variant (αC) labeled â¼130-kDa proteins in various brain regions. From immunohistochemistry data, both αA/B and αC exhibited diffuse labeling throughout brain, but αA/B labeling was more intracellular and punctate. Based on co-localization studies with antibodies to neuronal or astrocytic markers, αA/B labeled neurons in the pyramidal layer and dentate gyrus of the hippocampus, as well as cortex. αC labeled glia surrounding neurons (and possibly neurons) in the neuropil of the Purkinje cell layer of the cerebellum, the pyramidal cell layer and dentate gyrus of the hippocampus, and the cortex. According to electron microscopy data from the cerebellum, αA/B primarily labeled neurons intracellularly and αC labeled astrocytes at the plasma membrane. In summary, the B and C variants are the predominant NBCe1 variants in rat brain and exhibit different localization profiles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 155, Issue 3, 26 August 2008, Pages 818-832
Journal: Neuroscience - Volume 155, Issue 3, 26 August 2008, Pages 818-832
نویسندگان
D. Majumdar, A.B. Maunsbach, J.J. Shacka, J.B. Williams, U.V. Berger, K.P. Schultz, L.E. Harkins, W.F. Boron, K.A. Roth, M.O. Bevensee,