کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4342176 | 1295859 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Innervation and activity dependent dynamics of postsynaptic oxidative metabolism
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DPIProsperoafter egg layingAELTMRENMJTTXSynaptogenesisDMSO - DMSONeuromuscular junction - اتصال عصبی عضلانیtetramethylrhodamine ethyl ester - اتیل استر استرهای تترامتیل رودامینtetrodotoxin - تترو دوتوکسین analysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceImaging - تصویربرداریDiphenyleneiodonium - دیفنیلن اندونیمDimethylsulfoxide - دیمتیل سولفواکسیدMyosin heavy chain - زنجیره سنگین میوزینsyntaxin - سنتازینsyx - سکسCoefficient of Variation - ضریب تغییرProS - طرفدارانFlavoprotein - فلاوپروتئینAutofluorescence - فلوئورسانسMetabolism - متابولیسم MHC - مجموعه سازگاری بافتی اصلیMitochondria - میتوکندریاwild type - نوع وحشی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Despite extensive investigations into the mechanisms of aerobic respiration in mitochondria, the spontaneous metabolic activity of individual cells within a whole animal has not been observed in real time. Consequently, little is known about whether and how the level of mitochondrial energy metabolism is regulated in a cell during development of intact systems. Here we studied the dynamics of postsynaptic oxidative metabolism by monitoring the redox state of mitochondrial flavoproteins, an established indicator of energy metabolism, at the developing Drosophila neuromuscular junction. We detected transient and spatially synchronized flavoprotein autofluorescence signals in postsynaptic muscle cells. These signals were dependent on the energy substrates and coupled to changes in mitochondrial membrane potential and Ca2+ concentration. Notably, the rate of autofluorescence signals increased during synapse formation through contact with the motoneuronal axon. This rate was also influenced by the magnitude of synaptic inputs. Thus, presynaptic cells tightly regulate postsynaptic energy metabolism presumably to maintain an energetic balance during neuromuscular synaptogenesis. Our results suggest that flavoprotein autofluorescence imaging should allow us to begin assessing the progress of synapse formation from a metabolic perspective.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 152, Issue 1, 3 March 2008, Pages 40-49
Journal: Neuroscience - Volume 152, Issue 1, 3 March 2008, Pages 40-49
نویسندگان
H. Kazama, A. Ichikawa, H. Kohsaka, T. Morimoto-Tanifuji, A. Nose,