کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4349104 1296924 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Proton binding sites involved in the activation of acid-sensing ion channel ASIC2a
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Proton binding sites involved in the activation of acid-sensing ion channel ASIC2a
چکیده انگلیسی

Most acid-sensing ion channel (ASIC) subunits are activated by protons, but ASIC2b (a splice variant of ASIC2a) is acid-insensitive. Differences in protonatable residues between the extracellular loop regions of ASIC2a and ASIC2b may explain this difference. Site-directed mutagenesis, combined with immunocytochemistry and whole-cell patch clamp, demonstrated that mutating any one of five ASIC2a sites produces channels that traffic normally to the cell surface membrane but are insensitive to protons. One of the mutants forms functional heteromers with ASIC1a and ASIC2a, demonstrating that ion transport is intact in this mutant. These five sites may be involved in the activation of ASIC2a by protons.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 426, Issue 1, 9 October 2007, Pages 12–17
نویسندگان
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