کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4358593 1300443 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Methionine biosynthesis in Agrobacterium tumefaciens: study of the first enzyme
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Methionine biosynthesis in Agrobacterium tumefaciens: study of the first enzyme
چکیده انگلیسی

Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs in other bacteria, including Escherichia coli, the enzyme uses acetyl-CoA as a substrate and therefore is functionally a transacetylase. Moreover, the protein is thermolabile and the gene is under regulation of heat shock transcriptional activator σ32. 3. The gene has a SAM-riboswitch, which shuts off transcription by σ-32 as well as by the vegetative σ-70.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 164, Issue 1, January 2013, Pages 12–16
نویسندگان
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