کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4360852 | 1301319 | 2016 | 12 صفحه PDF | دانلود رایگان |
• Bacterial cell wall (CW) traverses the mouse placenta and is detected in the fetal brain
• CW induces transcription factor FoxG1 in the fetal cortex leading to neuroproliferation
• FoxG1 induction and neuroproliferation require TLR2 in vivo and in vitro
• CW exposure in utero results in abnormal postnatal cognitive behavior
SummaryMaternal infection during pregnancy is associated with adverse outcomes for the fetus, including postnatal cognitive disorders. However, the underlying mechanisms are obscure. We find that bacterial cell wall peptidoglycan (CW), a universal PAMP for TLR2, traverses the murine placenta into the developing fetal brain. In contrast to adults, CW-exposed fetal brains did not show any signs of inflammation or neuronal death. Instead, the neuronal transcription factor FoxG1 was induced, and neuroproliferation leading to a 50% greater density of neurons in the cortical plate was observed. Bacterial infection of pregnant dams, followed by antibiotic treatment, which releases CW, yielded the same result. Neuroproliferation required TLR2 and was recapitulated in vitro with fetal neuronal precursor cells and TLR2/6, but not TLR2/1, ligands. The fetal neuroproliferative response correlated with abnormal cognitive behavior in CW-exposed pups following birth. Thus, the bacterial CW-TLR2 signaling axis affects fetal neurodevelopment and may underlie postnatal cognitive disorders.
Graphical AbstractFigure optionsDownload high-quality image (117 K)Download as PowerPoint slide
Journal: - Volume 19, Issue 3, 9 March 2016, Pages 388–399