کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4361379 1301376 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Three-Dimensional Analysis of Budding Sites and Released Virus Suggests a Revised Model for HIV-1 Morphogenesis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Three-Dimensional Analysis of Budding Sites and Released Virus Suggests a Revised Model for HIV-1 Morphogenesis
چکیده انگلیسی

SummaryCurrent models of HIV-1 morphogenesis hold that newly synthesized viral Gag polyproteins traffic to and assemble at the cell membrane into spherical protein shells. The resulting late-budding structure is thought to be released by the cellular ESCRT machinery severing the membrane tether connecting it to the producer cell. Using electron tomography and scanning transmission electron microscopy, we find that virions have a morphology and composition distinct from late-budding sites. Gag is arranged as a continuous but incomplete sphere in the released virion. In contrast, late-budding sites lacking functional ESCRT exhibited a nearly closed Gag sphere. The results lead us to propose that budding is initiated by Gag assembly, but is completed in an ESCRT-dependent manner before the Gag sphere is complete. This suggests that ESCRT functions early in HIV-1 release—akin to its role in vesicle formation—and is not restricted to severing the thin membrane tether.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 4, Issue 6, 11 December 2008, Pages 592–599
نویسندگان
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