کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4361531 | 1301395 | 2009 | 15 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Nck- and N-WASP-Dependent Actin-Based Motility Is Conserved in Divergent Vertebrate Poxviruses Nck- and N-WASP-Dependent Actin-Based Motility Is Conserved in Divergent Vertebrate Poxviruses](/preview/png/4361531.png)
SummaryVaccinia virus enhances its cell-to-cell spread by stimulating actin polymerization via Src- and Abl-mediated phosphorylation of the highly conserved orthopoxvirus protein A36. The Yatapoxvirus, Yaba-like disease virus (YLDV), also induces actin polymerization, although it lacks an obvious A36 ortholog. We found that the YLDV protein YL126 can functionally replace A36 to promote Nck- and N-WASP-dependent actin polymerization. At least five phosphorylated tyrosines in YL126, rather than a single residue as in A36, are able to recruit Nck to promote actin polymerization. As is the case for A36, YL126-mediated actin tail formation is enhanced by the recruitment of Grb2 via a single phosphorylated tyrosine in YL126. Furthermore, highly divergent YL126 orthologs in Yaba monkey tumor, lumpy skin disease, Shope fibroma, myxoma, and swine and squirrel poxviruses also stimulate Nck- and N-WASP-dependent actin polymerization, suggesting that actin-based motility represents a common mechanism to enhance the cell-to-cell spread of vertebrate poxviruses.
Journal: - Volume 6, Issue 6, 17 December 2009, Pages 536–550