کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4361707 1301413 2009 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aldolase Is Essential for Energy Production and Bridging Adhesin-Actin Cytoskeletal Interactions during Parasite Invasion of Host Cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Aldolase Is Essential for Energy Production and Bridging Adhesin-Actin Cytoskeletal Interactions during Parasite Invasion of Host Cells
چکیده انگلیسی

SummaryApicomplexan parasites rely on actin-based motility to drive host cell invasion. Prior in vitro studies implicated aldolase, a tetrameric glycolytic enzyme, in coupling actin filaments to the parasite's surface adhesin microneme protein 2 (MIC2). Here, we test the essentiality of this interaction in host cell invasion. Based on in vitro studies and homology modeling, we generated a series of mutations in Toxoplasma gondii aldolase (TgALD1) that delineated MIC2 tail domain (MIC2t) binding function from its enzyme activity. We tested these mutants by complementing a conditional knockout of TgALD1. Mutations that affected glycolysis also reduced motility. Mutants only affecting binding to MIC2t had no motility phenotype, but were decreased in their efficiency of host cell invasion. Our studies demonstrate that aldolase is not only required for energy production but is also essential for efficient host cell invasion, based on its ability to bridge adhesin-cytoskeleton interactions in the parasite.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 5, Issue 4, 23 April 2009, Pages 353–364
نویسندگان
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